Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells.
نویسندگان
چکیده
منابع مشابه
Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells
We purified actin antibodies by affinity chromatography from the serum of rabbits immunized with glutaraldehyde-fixed chicken gizzard actin filaments and used this anti-actin to localize actin in myofibrils and fixed cultured cells at each stage of the cell cycle. By double immunodiffusion the anti-actin reacted with both smooth and skeletal muscle actin. Several blocking and absorption experim...
متن کاملActin in dividing cells: contractile ring filaments bind heavy meromyosin.
Many microfilaments and microtubules are well preserved after glycerol-extraction of HeLa cells at room temperature (22 degrees ). Incubation in heavy meromyosin from rabbit skeletal muscle results in conspicuous and characteristic "decoration" of microfilaments of the contractile ring. Decoration is completely prevented by 10 mM ATP or 2 mM pyrophosphate, and fails to occur if heavy meromyosin...
متن کاملFunction of Heavy Meromyosin in the Acceleration of Actin Polymerization.
The function of myosin A in the acceleration of actin polymerization was first observed by Laki and Clark (1) who noted the catalytic effect of myosin A. Martonosi and Gouvea (2) used a light scattering method to demonstrate that an enzymically active fragment of myosin A, heavy meromyosin, prepared by tryptic digestion, also accelerated the actin polymerization. Yagi and Sakakibara (3) have sh...
متن کاملThe Localization of Actin-like Fibers in Cultured Neuroblastoma Cells as Revealed by Heavy Meromyosin Binding
Actin-like protein has been isolated from cultured since normal cell morphology is disrupted during sympathetic ganglia of chick (6) and mammalian glycerination, the exact localization of actinbrain (18), and accounts for up to 207 of the total HMM complexes in axons and cell bodies has not cell protein (6) . The exact ultrastructural localizabeen ascertained . tion of such a large amount of ac...
متن کاملassessment of deep word knowledge in elementary and advanced iranian efl learners: a comparison of selective and productive wat tasks
testing plays a vital role in any language teaching program. it allows teachers and stakeholders, including program administrators, parents, admissions officers and prospective employers to be assured that the learners are progressing according to an accepted standard (douglas, 2010). the problems currently facing language testers have both practical and theoretical implications but the first i...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1979
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.80.3.509